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KMID : 1011620110270030001
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Korean Journal of Food and Cookey Science
2011 Volume.27 No. 3 p.1 ~ p.10
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Antioxidant Activity and Cytotoxic Effect of an Ethanol Extract from Seoritae
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전연희:Jeon Yeon-Hee
원지혜:Won Ji-Hye/권지은:Kwon Ji-Eun/김미라:Kim Mee-Ra
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Abstract
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The antioxidant activity and cytotoxic effect of an ethanol extract from Seoritae were analyzed to develop new functional food materials. The antioxidant activity of Seoritae was determined by measuring electron donating ability with 1,1-diphenyl-2picrylhydrazyl (DPPH) and 2-2"-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) assays, as well as the ferric reducing antioxidant power (FRAP) assay. The cytotoxic effect of the Seoritae ethanol extract was measured with the 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheltetrazolium (MTT) and sulforhodamine B (SRB) assays. As a result, the electron donating abilities of Seoritae against the DPPH and ABTS radicals were 63.75% and 87.68% at 500 μg/assay, respectively. The IC50 values of Seoritae in the DPPH and ABTS assays were 385.39 μg/assay (128.46 μg/mL) and 209.39 μg/assay (51.83 μg/mL). Additionally, the FRAP value of Seoritae was 0.84 FeSO4 eq. mM at 800 μg/assay. The total amounts of polyphenols and flavonoids, which indicate the antioxidant capability of Seoritae extract were 1.65 mg/g and 0.59 mg/g, respectively. Moreover, Seoritae extract showed a high cytotoxic effect of up to 81% against human cancer cells, particularly A-549 and HeLa cells. The growth inhibition rate of Seoritae extract against A-549 and HeLa cells was up to 76.48% and 75.67% in the MTT assay, and 78.98% and 80.54% in the SRB assay, respectively. The results of this study suggest that an ethanol extract of Seoritae is a potentially good natural antioxidant.
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KEYWORD
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antioxidant, cytotoxicity, phytochemical, Seoritae
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